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基于细胞的 sineup 非编码 rna 检测, 可专门增强 mrna 的翻译能力

JoVE Journal
Genetics

This content is Free Access.

JoVE Journal Genetics

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

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基于细胞的 sineup 非编码 rna 检测, 可专门增强 mrna 的翻译能力

8,072 Views

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10:21 min

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February 01, 2019

DOI:

10.3791/58627-v

DOI:

10.3791/58627-v

•

10:21 min

February 01, 2019

•

5 Views

Hazuki Takahashi*¹, Harshita Sharma*¹, Piero Carninci^1,2

¹Laboratory for Transcriptome Technology,RIKEN Center for Integrative Medical Sciences, ²TransSINE Technologies Co. Ltd.

Summary

sineup 是一种合成反义非编码 rna, 它包含一个结合域 (bd) 和一个效应域 (ed), 并向上调节目标 mrna 的翻译。在这里, 我们描述了在培养细胞系中检测 sineup 的方法, 分析了他们的翻译促进活性的西方印迹和半自动高通量成像系统。

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