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Immunisatie van alpaca's (Lama pacos) met eiwit antigenen en productie van antigeen-specifieke enkel domein antilichamen
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies
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Immunisatie van alpaca's (Lama pacos) met eiwit antigenen en productie van antigeen-specifieke enkel domein antilichamen

Nederlands

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22,596 Views

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05:27 min

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January 26, 2019

DOI:

10.3791/58471-v

DOI:
10.3791/58471-v

•

05:27 min
January 26, 2019

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18 Views
, , , , , , , ,
1Department of Molecular and Cellular Biochemistry,University of Kentucky, 2Division of Laboratory Animal Resources,University of Kentucky, 3River Hill Ranch

Summary

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Een methode voor de productie van één domein antilichamen van alpaca's, met inbegrip van immunisatie, bloedinzameling, B-cel isolatie en selectie wordt beschreven.

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Cite this Article

Chow, K. M., Whiteheart, S. W., Smiley, J. R., Sharma, S., Boaz, K., Coleman, M. J., Maynard, A., Hersh, L. B., Vander Kooi, C. W. Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies. J. Vis. Exp. (143), e58471, doi:10.3791/58471 (2019).

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