Journal
/
Biochemistry
/
Ekstracellulære Protein Microarray teknologi til High Throughput påvisning af lav affinitet Receptor-Ligand interaktioner
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Ekstracellulære Protein Microarray teknologi til High Throughput påvisning af lav affinitet Receptor-Ligand interaktioner

English

Automatically Generated

7,029 Views

•

06:01 min

•

January 07, 2019

DOI:

10.3791/58451-v

DOI:
10.3791/58451-v

•

06:01 min
January 07, 2019

•

5 Views
, , ,
1Receptor Discovery group, Microchemistry, Proteomics and Lipidomics Department,Genentech, 2Portfolio Management and Operations,Genentech

Summary

Automatically generated

Vi præsenterer her, en protokol til skærmen ekstracellulære protein microarrays for identifikation af roman receptor-ligand interaktioner i høj overførselshastighed. Vi beskriver også en metode til at forbedre afsløring af forbigående protein-protein interaktioner ved hjælp af protein-microbead komplekser.

Explore More Videos

Extracellular Protein MicroarrayHigh Throughput DetectionLow Affinity Receptor-ligand InteractionsProtein-protein InteractionsMicro ArrayerEpoxy Silane SlidesCy3 Labeled BSABlocking SolutionMultivalent ApproachFc-tagged Extracellular DomainsProtein A Coated MicrobeadsCy5 IgG Labeling

Read Article

Cite this Article

Husain, B., Paduchuri, S., Ramani, S. R., Martinez-Martin, N. Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions. J. Vis. Exp. (143), e58451, doi:10.3791/58451 (2019).

Download .ris file

Copy

Share Video

.

Copy