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Studium der Protein Import in Chloroplasten mit Protoplasten
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Biochemistry
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JoVE Journal Biochemistry
Studying Protein Import into Chloroplasts Using Protoplasts
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Studium der Protein Import in Chloroplasten mit Protoplasten

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9,377 Views

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06:29 min

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December 10, 2018

DOI:

10.3791/58441-v

DOI:
10.3791/58441-v

•

06:29 min
December 10, 2018

•

13 Views
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1Division of Integrative Biosciences and Biotechnology,Pohang University of Science and Technology, 2Department of Life Sciences,Pohang University of Science and Technology

Summary

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Hier beschreiben wir ein Protokoll, um Proteine in Protoplasten zum Ausdruck zu bringen, mit PEG-vermittelten Transformationsmethode. Die Methode bietet einfach Ausdruck der interessierenden Proteine und effiziente Untersuchung von Protein-Lokalisierung und der Importvorgang für verschiedenen experimentellen Bedingungen in-vivo.

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Cite this Article

Lee, J., Kang, H., Hwang, I. Studying Protein Import into Chloroplasts Using Protoplasts. J. Vis. Exp. (142), e58441, doi:10.3791/58441 (2018).

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