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Immunohistochemische detectie van 5-Methylcytosine en 5-Hydroxymethylcytosine bij de ontwikkeling en Postmitotic muis Retina
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
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Immunohistochemische detectie van 5-Methylcytosine en 5-Hydroxymethylcytosine bij de ontwikkeling en Postmitotic muis Retina

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8,657 Views

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07:50 min

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August 29, 2018

DOI:

10.3791/58274-v

DOI:
10.3791/58274-v

•

07:50 min
August 29, 2018

•

1 Views
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1BioTime, Inc

Summary

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Het doel van dit verslag is om te beschrijven van het protocol voor robuuste immunohistochemische detectie van epigenetische markers, 5-methylcytosine (5mC) en 5-hydroxymethylcytosine (5hmC) bij de ontwikkeling en postmitotic muis netvlies.

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Cite this Article

Singh, R. K., Diaz, P. E., Binette, F., Nasonkin, I. O. Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina. J. Vis. Exp. (138), e58274, doi:10.3791/58274 (2018).

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