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September 19, 2018
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Summary
Automatically generated
在这里, 我们提出了一个方法, 以产生组织特定的二进制转录系统的果蝇, 取代第一编码外显子的基因与转录驱动。CRISPR/Cas9-based 方法在被替换的基因的内生调控下放置一个 transactivator 序列, 从而促进 transctivator 表达专门在基因特定的时空模式。
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Du, L., Zhou, A., Sohr, A., Roy, S. An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing. J. Vis. Exp. (139), e58268, doi:10.3791/58268 (2018).
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