Journal
/
Immunology and Infection
/
Humaniseret SCID-NOD-IL2rγnull (hu-NSG) mus Model for HIV replikation og Latency undersøgelser
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Humaniseret SCID-NOD-IL2rγnull (hu-NSG) mus Model for HIV replikation og Latency undersøgelser

English

Automatically Generated

15,308 Views

•

07:10 min

•

January 07, 2019

DOI:

10.3791/58255-v

DOI:
10.3791/58255-v

•

07:10 min
January 07, 2019

•

9 Views
, , , ,
1Department of Molecular and Cellular Biology,Beckman Research Institute of City of Hope, 2Irell and Manela Graduate School of Biological Sciences,Beckman Research Institute of City of Hope

Summary

Automatically generated

Denne protokol indeholder en metode til at etablere humaniseret mus (hu-NSG) via intrahepatisk injektion af menneskelige hæmatopoietisk stamceller i stråling-aircondition neonatal NSG mus. Hu-NSG musen er modtagelige for HIV-infektion og kombinatorisk antiretroviral behandling (vogn) og tjener som en passende patofysiologiske model for HIV replikation og latency undersøgelser.

Explore More Videos

Hu-NSG Mouse ModelHIV ReplicationHIV LatencyNon-invasiveHuman Stem CellsCD34+ HSCsNOD/SCID/IL2rγnull MiceCARTHIV InfectionEngraftment ValidationFlow Cytometry

Read Article

Cite this Article

Xia, X., Li, H., Satheesan, S., Zhou, J., Rossi, J. J. Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies. J. Vis. Exp. (143), e58255, doi:10.3791/58255 (2019).

Download .ris file

Copy

Share Video

.

Copy