We describe a method to engineer a retinal tissue composed of retinal pigment epithelial cells derived from human pluripotent stem cells cultured on top of human amniotic membranes and its preparation for grafting in animal models.
Ben M'Barek, K., Habeler, W., Plancheron, A., Jarraya, M., Goureau, O., Monville, C. Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells. J. Vis. Exp. (139), e58216, doi:10.3791/58216 (2018).