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Kafein ayıklama, enzimatik aktivite ve kafein Synthase bitki hücre kullanılamaz hale gelen gen ifadesi
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Biochemistry
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JoVE Journal Biochemistry
Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions
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Kafein ayıklama, enzimatik aktivite ve kafein Synthase bitki hücre kullanılamaz hale gelen gen ifadesi

Türkçe

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12,384 Views

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09:11 min

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October 02, 2018

DOI:

10.3791/58166-v

DOI:
10.3791/58166-v

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09:11 min
October 02, 2018

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17 Views
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1Unidad de Bioquímica y Biología Molecular de Plantas,Centro de Investigación Científica de Yucatán, 2CONACYT, Facultad de Ingeniería Química, Campus de Ciencias Exactas e Ingeniería,Universidad Autónoma de Yucatán

Summary

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Bu iletişim kuralı çıkarma ve miktar hücre süspansiyonlar C. arabica L., kafein için verimli bir metodoloji ve kafein synthase enzimatik aktivite ile ifade düzeyini değerlendirmek için deneysel bir işlem açıklar Bu enzimi kodlayan gen.

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Pech-Kú, R., Muñoz-Sánchez, J. A., Monforte-González, M., Vázquez-Flota, F., Rodas-Junco, B. A., Hernández-Sotomayor, S. T. Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions. J. Vis. Exp. (140), e58166, doi:10.3791/58166 (2018).

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