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Kwantificering van Efferocytosis door eencellige fluorescentie microscopie
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
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Kwantificering van Efferocytosis door eencellige fluorescentie microscopie

Nederlands

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12,187 Views

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06:15 min

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August 18, 2018

DOI:

10.3791/58149-v

DOI:
10.3791/58149-v

•

06:15 min
August 18, 2018

•

15 Views
, , ,
1Department of Microbiology and Immunology and the Center for Human Immunology,University of Western Ontario, 2Institute of Infection and Global Health,University of Liverpool, 3Department of Respiratory Research,Aintree University Hospital NHS Foundation Trust

Summary

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Efferocytosis, de fagocytische verwijdering van apoptotic cellen, is nodig om de homeostase te handhaven en wordt vergemakkelijkt door de receptoren en signaalroutes waarmee voor de erkenning, de engulfment, en de internalisering van de apoptotic cellen. Hierin presenteren wij een fluorescentie microscopie protocol voor de kwantificering van efferocytosis en de activiteit van efferocytic signaalroutes.

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Cite this Article

Taruc, K., Yin, C., Wootton, D. G., Heit, B. Quantification of Efferocytosis by Single-cell Fluorescence Microscopy. J. Vis. Exp. (138), e58149, doi:10.3791/58149 (2018).

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