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用 RNA原位杂交法对形态学组织环境中的遗传变异、短靶点和突变进行可视化
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August 14, 2018
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Genetic VariantsShort TargetsPoint MutationsRNA In Situ HybridizationCell Type ExpressionSplice VariantsNeuroscienceOncologyExon JunctionsSensitivitySpecificitySingle CellsFormalin FixationParaffin EmbeddingMicrotome SectioningTarget RetrievalWash BufferHematoxylin CounterstainProbe HybridizationAmplification Reagents
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Anderson, C. M., Laeremans, A., Wang, X. M., Wu, X., Zhang, B., Doolittle, E., Kim, J., Li, N., Pimentel, H. X. Y., Park, E., Ma, X. Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay. J. Vis. Exp. (138), e58097, doi:10.3791/58097 (2018).
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