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Isolering af retinale arterioler for Ex Vivo celle fysiologi undersøgelser
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JoVE Journal Biology
Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies

Isolering af retinale arterioler for Ex Vivo celle fysiologi undersøgelser

DOI:
10.3791/57944-v

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12:42 min

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July 14, 2018

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, , , , , , , ,
1Centre for Experimental Medicine,Queen’s University of Belfast, 2Centre for Biomedical Sciences (Education),Queen’s University of Belfast, 3Department of Pharmaceutical Chemistry and Pharmacognosy,Naresuan University, 4School of Medicine, Dentistry and Biomedical Sciences,Queen’s University of Belfast

Chapters

  • 00:00Title
  • 01:23Isolation of Retinal Arterioles
  • 05:09Arteriolar Pressure Myography
  • 07:22Patch-Clamp Electrophysiology
  • 09:58Results
  • 12:00Conclusion

Summary

Automatic Translation

Automatic Translation

Dette manuskript beskriver en enkel protokol til isolering af arterioler fra rotte nethinden, der kan bruges i elektrofysiologiske, calcium imaging og pres blodkar undersøgelser.

Tags

Keywords: Retinal ArteriolesRetinal Blood FlowRetinal PerfusionVascular Smooth MusclePatch ClampCalcium ImagingPressure MyographyRetinal DiseasesDiabetic RetinopathyGlaucomaBranch Vein OcclusionsEye DissectionRetina IsolationLow Calcium Hanks’ Solution

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