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改良多向分化效力的常规人多能干细胞的化学逆转与幼稚样态
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency
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改良多向分化效力的常规人多能干细胞的化学逆转与幼稚样态

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9,721 Views

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09:07 min

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June 10, 2018

DOI:

10.3791/57921-v

DOI:
10.3791/57921-v

•

09:07 min
June 10, 2018

•

1 Views
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1Department of Oncology, Division of Pediatric Oncology and Institute for Cell Engineering,Johns Hopkins School of Medicine

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Chemical ReversionConventional Human Pluripotent Stem CellsNaive-like StateMultilineage DifferentiationLIF-3i MethodPre-implantation EpiblastLineage-primed Gene ExpressionEpigenomic StabilityRegenerative MedicineHPSC DifferentiationGene TargetingInterspecies ChimerasLIF-5i MediumCell DissociationFeeder-free Culture

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Park, T. S., Zimmerlin, L., Evans-Moses, R., Zambidis, E. T. Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency. J. Vis. Exp. (136), e57921, doi:10.3791/57921 (2018).

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