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Contrast-Matching wasmiddel in Small-Angle Neutron Scattering experimenten voor membraan eiwit structurele analyse en modellering van de Ab Initio
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Biochemistry
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JoVE Journal Biochemistry
Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
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Contrast-Matching wasmiddel in Small-Angle Neutron Scattering experimenten voor membraan eiwit structurele analyse en modellering van de Ab Initio

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12,117 Views

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10:27 min

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October 21, 2018

DOI:

10.3791/57901-v

DOI:
10.3791/57901-v

•

10:27 min
October 21, 2018

•

2 Views
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1Neutron Scattering Division,Oak Ridge National Laboratory, 2School of Chemistry and Biochemistry,Georgia Institute of Technology

Summary

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Dit protocol laat zien hoe een model met een lage resolutie ab initio en structurele details van een wasmiddel-ontbindend membraan eiwit in small-angle neutron scattering met contrast-matching van het detergent oplossing te verkrijgen.

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Oliver, R. C., Naing, S., Weiss, K. L., Pingali, S. V., Lieberman, R. L., Urban, V. S. Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling. J. Vis. Exp. (140), e57901, doi:10.3791/57901 (2018).

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