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Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals
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Immunology and Infection
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JoVE Journal Immunology and Infection
Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

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6,740 Views

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08:49 min

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February 14, 2019

DOI:

10.3791/57662-v

DOI:
10.3791/57662-v

•

08:49 min
February 14, 2019

•

1 Views
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1Infectious Pathology and Transplantation Division,Bellvitge Biomedical Research Institute (IDIBELL), 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (IBCh),Russian Academy of Sciences, 3Semiotik LLC, 4Auckland University of Technology, 5Intensive Care Department,Bellvitge University Hospital

Summary

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This work shows the potential of printed glycan array (PGA) technology for the analysis of circulating anti-carbohydrate antibodies in small animals.

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Glycan ArrayAnti-carbohydrate AntibodiesSmall AnimalsSerumPrinted Glycan ArrayGlycan TargetsPolysaccharidesPBSN-hydroxy 6 Cinnamic Derivatized Glass SlidesBlocking BufferBufferMice SerumImmunoglobulin AggregationCentrifugationIncubation Chamber

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Olivera-Ardid, S., Khasbiullina, N., Nokel, A., Formanovsky, A., Popova, I., Tyrtysh, T., Kunetskiy, R., Shilova, N., Bovin, N., Bello-Gil, D., Mañez, R. Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals. J. Vis. Exp. (144), e57662, doi:10.3791/57662 (2019).

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