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Polysome profilering i Leishmania, menneskelige celler og mus Testis
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Biochemistry
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JoVE Journal Biochemistry
Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Polysome profilering i Leishmania, menneskelige celler og mus Testis

English

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17,396 Views

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14:32 min

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April 08, 2018

DOI:

10.3791/57600-v

DOI:
10.3791/57600-v

•

14:32 min
April 08, 2018

•

9 Views
, , , , , , , , ,
1Department of Cell Biology and Biochemistry,Texas Tech University Health Sciences Center, 2Department of Biological Sciences,Texas Tech University, 3CISER (Center for the Integration of STEM Education & Research),Texas Tech University

Summary

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Det overordnede mål med polysome profilering teknik er analyse af translationel aktivitet af individuelle mRNAs eller transkriptom mRNAs under proteinsyntesen. Metoden er vigtigt, at undersøgelser af protein syntese forordning, oversættelse aktivering og undertrykkelse i sundhed og flere sygdomme hos mennesker.

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Cite this Article

Karamysheva, Z. N., Tikhonova, E. B., Grozdanov, P. N., Huffman, J. C., Baca, K. R., Karamyshev, A., Denison, R. B., MacDonald, C. C., Zhang, K., Karamyshev, A. L. Polysome Profiling in Leishmania, Human Cells and Mouse Testis. J. Vis. Exp. (134), e57600, doi:10.3791/57600 (2018).

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