/
/
CARIP-Seq e ChIP-Seq: metodi per identificare la cromatina associata RNAs e interazioni proteina-DNA in cellule staminali embrionali
A subscription to JoVE is required to view this content. Sign in or start your free trial.
9,651 Views
•
11:13 min
•
May 25, 2018
DOI:
Summary
Automatically generated
Qui, descriviamo i metodi per eseguire ChIP-Seq e CARIP-Seq, compresa la preparazione di libreria per il sequenziamento di nuova generazione, per generare globale epigenomic e cromatina-associated RNA mappe in cellule ES.
Explore More Videos
CARIP-SeqChIP-SeqChromatin-associated RNAsProtein-DNA InteractionsEmbryonic Stem CellsEpigeneticsTranscription FactorsEpigenetic RegulatorsHistone ModificationsChromatin ImmunoprecipitationSonicationFormaldehyde Cross-linkingGlycinePhosphate Buffered SalineTrypsinizationMEF MediumFetal Bovine SerumSonication BufferSodium Dodecyl Sulfate
Read Article
Cite this Article
Kidder, B. L. CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells. J. Vis. Exp. (135), e57481, doi:10.3791/57481 (2018).
Copy