Journal
/
Developmental Biology
/
הערכת Alkalinization Lysosomal ב המעי של חיים Caenorhabditis elegans
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
Please note that all translations are automatically generated. Click here for the English version.

הערכת Alkalinization Lysosomal ב המעי של חיים Caenorhabditis elegans

עברית

Automatically Generated

5,848 Views

•

06:18 min

•

April 13, 2018

DOI:

10.3791/57414-v

DOI:
10.3791/57414-v

•

06:18 min
April 13, 2018

•

3 Views
,
1Department of Biology,University of Saskatchewan

Summary

Automatically generated

מדריך צעד אחר צעד כדי לחקור אובדן של חומציות lysosomal במעי של C. elegans באמצעות לצבוע חיוני רגיש pH 5 (6) - carboxy - 2', 7'-dichlorofluorescein diacetate (cDCFDA)

Explore More Videos

Lysosomal AlkalinizationCaenorhabditis ElegansAutophagyLysosomal FunctionProtein DegradationCDCFDA StainingOP50 BacteriaNGM PlatesM9 BufferAgarose PadSodium Azide

Read Article

Cite this Article

Baxi, K., de Carvalho, C. E. Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans. J. Vis. Exp. (134), e57414, doi:10.3791/57414 (2018).

Download .ris file

Copy

Share Video

.

Copy