Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

Lorenzo Brunetti; Michael C. Gundry; Ayumi Kitano; Daisuke Nakada; Margaret A. Goodell

doi:10.3791/57278

Journal

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Genetics

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CRISPR/Cas9 によるマウスおよび人間の造血前駆細胞の高効率遺伝子破壊

JoVE Journal
Genetics

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JoVE Journal Genetics

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

Please note that all translations are automatically generated. Click here for the English version.

CRISPR/Cas9 によるマウスおよび人間の造血前駆細胞の高効率遺伝子破壊

13,039 Views

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08:27 min

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April 10, 2018

DOI:

10.3791/57278-v

DOI:

10.3791/57278-v

•

08:27 min

April 10, 2018

•

6 Views

Lorenzo Brunetti*^1,2,3, Michael C. Gundry*^1,2,4, Ayumi Kitano⁴, Daisuke Nakada^1,2,4, Margaret A. Goodell^1,2,4,5

¹Stem Cells & Regenerative Medicine Center,Baylor College of Medicine, ²Center for Cell and Gene Therapy,Baylor College of Medicine, ³Centro di Ricerca Emato-Oncologica (CREO),University of Perugia, ⁴Department of Molecular & Human Genetics,Baylor College of Medicine, ⁵Texas Children’s Hospital & Houston Methodist Hospital

Summary

高速のためのプロトコルマウスに CRISPR/Cas9 を介した遺伝子破壊とひと造血細胞はこの資料に記載されています。Cas9 sgRNA ribonucleoproteins は、sgRNAsの in vitro転写と商業 Cas9 を介して生成されたとエレクトロポレーションを介して紹介しています。限られた時間と金銭的コスト高の編集効率を実現します。

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CRISPR/Cas9 によるマウスおよび人間の造血前駆細胞の高効率遺伝子破壊

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Summary

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