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人端粒酶逆转录蛋白 rna 依赖性 rna 聚合酶活性的半定量检测
JoVE Journal
Genetics
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JoVE Journal Genetics
Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
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人端粒酶逆转录蛋白 rna 依赖性 rna 聚合酶活性的半定量检测

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08:26 min

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June 12, 2018

DOI:

10.3791/57021-v

DOI:
10.3791/57021-v

•

08:26 min
June 12, 2018

•

6 Views
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1Division of Cancer Stem Cell,National Cancer Center Research Institute

Tags

RNA-dependent RNA PolymeraseTERTRNA BiologyRdRP ActivityHeLa CellsCell LysisImmunoprecipitationProtein PurificationMicrococcal NucleaseSemi-quantitative Detection

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Maida, Y., Yasukawa, M., Ghilotti, M., Ando, Y., Masutomi, K. Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein. J. Vis. Exp. (136), e57021, doi:10.3791/57021 (2018).

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