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On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
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February 23, 2018
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Rapid and accurate detection of plant pathogens on-site, especially soil-borne pathogens, is crucial to prevent further inoculum production and proliferation of plant diseases in the field. The method developed here using a portable real-time PCR detection system enables on-site diagnosis under field conditions.
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DeShields, J. B., Bomberger, R. A., Woodhall, J. W., Wheeler, D. L., Moroz, N., Johnson, D. A., Tanaka, K. On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System. J. Vis. Exp. (132), e56891, doi:10.3791/56891 (2018).
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