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08:45 min
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August 22, 2018
DOI:
1Division of Experimental Hematology,Cincinnati Children’s Hospital Medical Center, 2Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute,Perelman School of Medicine, University of Pennsylvania, 3Department of Obstetrics and Gynecology, Southwest Hospital,Third Military Medical University
Summary
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しみが付くプロトコル標準ウエスタンとして 500 造血幹前駆細胞の数を分析するため最適化を行った。最適化を含むセルのサンプルの処理、管の転送を制限して塩化物イオンとサンプル バッファー内セルを直接溶解に注意してください。
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Cite this Article
Cai, X., Zheng, Y., Speck, N. A. A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells. J. Vis. Exp. (138), e56855, doi:10.3791/56855 (2018).
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