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Un Western Blotting protocole pour un petit nombre de cellules souches hématopoïétiques
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08:45 min
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August 22, 2018
DOI:
1Division of Experimental Hematology,Cincinnati Children’s Hospital Medical Center, 2Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute,Perelman School of Medicine, University of Pennsylvania, 3Department of Obstetrics and Gynecology, Southwest Hospital,Third Military Medical University
Summary
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Un standard Western blotting protocole a été optimisé pour analyser aussi peu que 500 souches hématopoïétiques ou de cellules progénitrices. Optimisation implique attention manipulation de l’échantillon cellulaire, limiter les transferts entre les tubes et lyse directement les cellules de la mémoire tampon de Laemmli témoin.
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Cite this Article
Cai, X., Zheng, Y., Speck, N. A. A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells. J. Vis. Exp. (138), e56855, doi:10.3791/56855 (2018).
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