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Generation af standardiserede og reproducerbare forhjernen-type Cerebral Organoids fra menneskelige induceret pluripotente stamceller
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

Generation af standardiserede og reproducerbare forhjernen-type Cerebral Organoids fra menneskelige induceret pluripotente stamceller

DOI:
10.3791/56768-v

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10:25 min

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January 23, 2018

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, , , ,
1Institute of Reconstructive Neurobiology,University of Bonn, 2Central Institute of Mental Health,University of Heidelberg/Medical Faculty Mannheim, 3Hector Institute for Translational Brain Research (HITBR gGmbH)

Chapters

  • 00:05Title
  • 00:55iPSC Aggregate Generation
  • 02:30Anterior Neuroectoderm Induction
  • 03:51Neuroectodermal Aggregates embedding within a Matrix Scaffold
  • 05:49Forebrain-type Organoid Generation, Fixation, and Validation
  • 07:58Results: Representative Forebrain-type Organoid Validation
  • 09:36Conclusion

Summary

Automatic Translation

Automatic Translation

Cerebral organoids repræsenterer en nye modelsystem til at undersøge tidlig menneskelige hjerne udvikling i vitro. Denne artikel giver den detaljeret metode til effektivt generere homogene dorsale forhjernen-type organoids fra menneskelige inducerede pluripotente stamceller herunder kritiske karakterisering og validering trin.

Tags

Human Induced Pluripotent Stem CellsForebrain-type Cerebral OrganoidsStandardized And Reproducible Organoid GenerationModeling NeurodevelopmentCortical GenesisAggregationAnterior Neuroectoderm Induction

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