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Lavorazione di tessuto cardiaco umano verso la matrice extracellulare autoassemblanti idrogel per In Vitro e In Vivo applicazioni
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December 04, 2017
DOI:
1Charité – Universitätsmedizin Berlin,corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2Berlin-Brandenburg Center for Regenerative Therapies (BCRT), 3German Center for Cardiovascular Research (DZHK), 4Deutsches Herzzentrum Berlin (DHZB), 5Department of Cardiovascular Surgery, Charité – Universitätsmedizin Berlin,corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health
Summary
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Questo protocollo descrive la completa decellularizzazione di miocardio umano pur conservando le sue componenti della matrice extracellulare. Ulteriore elaborazione dei risultati nella produzione di microparticelle e un idrogel autoassemblanti cytoprotective matrice extracellulare.
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Cite this Article
Becker, M., Maring, J. A., Oberwallner, B., Kappler, B., Klein, O., Falk, V., Stamm, C. Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications. J. Vis. Exp. (130), e56419, doi:10.3791/56419 (2017).
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