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Absorption of Nasal and Bronchial Fluids: Precision Sampling of the Human Respiratory Mucosa and Laboratory Processing of Samples
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Medicine
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JoVE Journal Medicine
Absorption of Nasal and Bronchial Fluids: Precision Sampling of the Human Respiratory Mucosa and Laboratory Processing of Samples

Absorption of Nasal and Bronchial Fluids: Precision Sampling of the Human Respiratory Mucosa and Laboratory Processing of Samples

English

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28,241 Views

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11:54 min

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January 21, 2018

DOI:

10.3791/56413-v

DOI:
10.3791/56413-v

•

11:54 min
January 21, 2018

•

60 Views
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1National Heart and Lung Institute, Faculty of Medicine, Imperial College London,St Mary’s Hospital, 2St Mary’s Hospital,Imperial College Healthcare Trust

Summary

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This manuscript describes the use of nasal and bronchial absorption techniques, specifically using synthetic absorptive matrices (SAM) to sample the mucosal lining fluid (MLF) of the upper and lower airway. These methods provide better standardization and tolerability than existing respiratory sampling techniques.

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Cite this Article

Thwaites, R. S., Jarvis, H. C., Singh, N., Jha, A., Pritchard, A., Fan, H., Tunstall, T., Nanan, J., Nadel, S., Kon, O. M., Openshaw, P. J., Hansel, T. T. Absorption of Nasal and Bronchial Fluids: Precision Sampling of the Human Respiratory Mucosa and Laboratory Processing of Samples. J. Vis. Exp. (131), e56413, doi:10.3791/56413 (2018).

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