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Estabelecimento de Zebrafish Larval como um modelo Animal para investigar Trypanosoma cruzi motilidade In Vivo
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September 30, 2017
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1Laboratory of Neurosciences and Circadian Rhythms, School of Medicine,Universidad de los Andes, 2Biophysics Group, Department of Physics,Universidad de los Andes, 3Laboratory of Basic Medical Sciences, School of Medicine,Universidad de los Andes, 4Department of Microbiology and Immunology,University of North Carolina, 5Notre Dame Initiative for Global Development,University of Notre Dame, 6USAID Research and Innovation Fellowship program
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Neste protocolo, fluorescente etiquetadas T. cruzi foram injetados em larvas de peixe-zebra transparente, e motilidade do parasita foi observada na vivo usando microscopia de fluorescência folha de luz.
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Akle, V., Agudelo-Dueñas, N., Molina-Rodriguez, M. A., Kartchner, L. B., Ruth, A. M., González, J. M., Forero-Shelton, M. Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo. J. Vis. Exp. (127), e56238, doi:10.3791/56238 (2017).
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