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一本鎖 Rna の二重鎖 Rna の特定のシーケンスで選択的な認識化学修飾ペプチド核酸
JoVE Journal
Genetics
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JoVE Journal Genetics
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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一本鎖 Rna の二重鎖 Rna の特定のシーケンスで選択的な認識化学修飾ペプチド核酸

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9,168 Views

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09:04 min

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September 21, 2017

DOI:

10.3791/56221-v

DOI:
10.3791/56221-v

•

09:04 min
September 21, 2017

•

5 Views
, ,
1Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences,Nanyang Technological University

Summary

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今回は合成のためのプロトコル、ペプチド核酸 (PNA) オリゴマーを組み込むの精製変更残基。PNAs の変更によって RNA 二重鎖の認識の特性の生化学的および生物物理学的方法を説明します。

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Cite this Article

Toh, D. K., Patil, K. M., Chen, G. Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids. J. Vis. Exp. (127), e56221, doi:10.3791/56221 (2017).

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