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Riconoscimento di sequenza-specifico e selettivo di Double-stranded RNA sopra Single-stranded RNA da modificati chimicamente acidi peptido nucleici
JoVE Journal
Genetics
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JoVE Journal Genetics
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Riconoscimento di sequenza-specifico e selettivo di Double-stranded RNA sopra Single-stranded RNA da modificati chimicamente acidi peptido nucleici

italiano

Automatically Generated

9,168 Views

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09:04 min

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September 21, 2017

DOI:

10.3791/56221-v

DOI:
10.3791/56221-v

•

09:04 min
September 21, 2017

•

5 Views
, ,
1Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences,Nanyang Technological University

Summary

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Segnaliamo i protocolli per la sintesi e purificazione degli oligomeri di Peptide degli acidi nucleici (PNA) che incorporano per volta residui. Sono descritti i metodi biochimici e biofisici per la caratterizzazione del riconoscimento del RNA duplex dal PNAs modificate.

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Cite this Article

Toh, D. K., Patil, K. M., Chen, G. Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids. J. Vis. Exp. (127), e56221, doi:10.3791/56221 (2017).

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