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Volgorde-specifieke en selectieve erkenning van Double-stranded RNAs over Single-stranded RNAs van chemisch gewijzigd Peptide nucleïnezuren
JoVE Journal
Genetics
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JoVE Journal Genetics
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Volgorde-specifieke en selectieve erkenning van Double-stranded RNAs over Single-stranded RNAs van chemisch gewijzigd Peptide nucleïnezuren

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9,168 Views

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09:04 min

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September 21, 2017

DOI:

10.3791/56221-v

DOI:
10.3791/56221-v

•

09:04 min
September 21, 2017

•

5 Views
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1Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences,Nanyang Technological University

Summary

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We melden de protocollen voor de synthese en zuivering van Peptide Nucleic Acid (PNA) oligomeren opnemen gewijzigd residuen. De biochemische en Biofysische methoden voor de karakterisatie van de erkenning van RNA duplexen door de gewijzigde PNAs worden beschreven.

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Cite this Article

Toh, D. K., Patil, K. M., Chen, G. Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids. J. Vis. Exp. (127), e56221, doi:10.3791/56221 (2017).

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