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蚊幼虫における遺伝子の機能解析の遺伝子配達ベクトルとして組換え蚊 Densovirus を使用
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Developmental Biology
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JoVE Journal Developmental Biology
Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae
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蚊幼虫における遺伝子の機能解析の遺伝子配達ベクトルとして組換え蚊 Densovirus を使用

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6,588 Views

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12:30 min

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October 06, 2017

DOI:

10.3791/56121-v

DOI:
10.3791/56121-v

•

12:30 min
October 06, 2017

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2 Views
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1Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health,Southern Medical University, 2Reproductive Medical Centre of Guangdong Women and Children’s Hospital

Summary

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良品組換えネッタイシマカdensovirus (AaeDV)生体内での配信システムを開発する、人工イントロン小 RNA 発現戦略を使用して報告します。建設、パッケージング、および幼虫の感染に関しては同様に rAaeDV のベクトルの定量分析のための詳細な手順を説明します。

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Cite this Article

Liu, P., Xu, J., Dong, Y., Chen, X., Gu, J. Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae. J. Vis. Exp. (128), e56121, doi:10.3791/56121 (2017).

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