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Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction
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Neuroscience
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JoVE Journal Neuroscience
Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction

Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction

English

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11,276 Views

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08:11 min

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August 25, 2017

DOI:

10.3791/55994-v

DOI:
10.3791/55994-v

•

08:11 min
August 25, 2017

•

3 Views
, , , ,
1Department of Pharmacology, Toxicology, and Neuroscience,Louisiana State University Health Sciences Center, 2Department of Molecular & Integrative Physiology,University of Kansas Medical Center

Summary

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Methods for a wide-scale central nervous system gene delivery in the rat are covered. In this example, the purpose is to mimic a disease that affects the entire spinal cord. The widespread transduction can be used to deliver a therapeutic protein to the CNS from a one-time, peripheral administration.

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Cite this Article

Grames, M. S., Jackson, K. L., Dayton, R. D., Stanford, J. A., Klein, R. L. Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction. J. Vis. Exp. (126), e55994, doi:10.3791/55994 (2017).

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