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体外受精卵の Live 細胞イメージング偏光とシロイヌナズナの胚パターン形成の胚珠培養
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Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
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体外受精卵の Live 細胞イメージング偏光とシロイヌナズナの胚パターン形成の胚珠培養

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10,720 Views

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05:41 min

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September 11, 2017

DOI:

10.3791/55975-v

DOI:
10.3791/55975-v

•

05:41 min
September 11, 2017

•

32 Views
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1Division of Biological Science, Graduate School of Science,Nagoya University, 2Higashiyama Live-Holonics Project, JST-ERATO,Nagoya University, 3Institute of Transformative Bio-Molecules (ITbM),Nagoya University

Summary

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本稿では体外胚珠栽培手法についてシロイヌナズナ受精および胚の住セルイメージ投射を可能にします。このメソッドを利用して、受精卵の偏波の中に細胞内動態と初期胚細胞運命決定を可視化します。

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Cite this Article

Kurihara, D., Kimata, Y., Higashiyama, T., Ueda, M. In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana. J. Vis. Exp. (127), e55975, doi:10.3791/55975 (2017).

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