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In Vitro Zaadknop teelt voor Live-cel Imaging van Zygote polarisatie en Embryo patronen in Arabidopsis thaliana
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Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
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In Vitro Zaadknop teelt voor Live-cel Imaging van Zygote polarisatie en Embryo patronen in Arabidopsis thaliana

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10,720 Views

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05:41 min

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September 11, 2017

DOI:

10.3791/55975-v

DOI:
10.3791/55975-v

•

05:41 min
September 11, 2017

•

32 Views
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1Division of Biological Science, Graduate School of Science,Nagoya University, 2Higashiyama Live-Holonics Project, JST-ERATO,Nagoya University, 3Institute of Transformative Bio-Molecules (ITbM),Nagoya University

Summary

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Dit manuscript beschrijft een in vitro zaadknop cultivatiemethode die het mogelijk live-cel beeldvorming van Arabidopsis zygoten en embryo's maakt. Deze methode wordt gebruikt om te visualiseren de intracellulaire dynamiek tijdens de zygote polarisatie en de cel lot specificatie bij de ontwikkeling van de embryo's.

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Cite this Article

Kurihara, D., Kimata, Y., Higashiyama, T., Ueda, M. In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana. J. Vis. Exp. (127), e55975, doi:10.3791/55975 (2017).

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