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Uma molécula simples, robusto e alta taxa de transferência de fluxo alongamento do ensaio implementação para estudar o transporte de moléculas ao longo de DNA
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Uma molécula simples, robusto e alta taxa de transferência de fluxo alongamento do ensaio implementação para estudar o transporte de moléculas ao longo de DNA

português

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7,834 Views

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12:05 min

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October 01, 2017

DOI:

10.3791/55923-v

DOI:
10.3791/55923-v

•

12:05 min
October 01, 2017

•

4 Views
,
1Broad Institute,Massachusetts Institute of Technology and Harvard Medical School, 2Dept. of Biological Engineering,Massachusetts Institute of Technology

Summary

Automatically generated

Este protocolo demonstra um ensaio de alongamento de fluxo única molécula simples, robusta e de alta taxa de transferência para estudar a difusão unidimensional (1D) moléculas ao longo do DNA.

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Cite this Article

Xiong, K., Blainey, P. C. A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA. J. Vis. Exp. (128), e55923, doi:10.3791/55923 (2017).

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