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Einfach, Robust und hohen Durchsatz Molekül fließen Stretching Assay-Implementierung für das Studium Transport von Molekülen entlang DNA
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
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Einfach, Robust und hohen Durchsatz Molekül fließen Stretching Assay-Implementierung für das Studium Transport von Molekülen entlang DNA

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7,834 Views

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12:05 min

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October 01, 2017

DOI:

10.3791/55923-v

DOI:
10.3791/55923-v

•

12:05 min
October 01, 2017

•

4 Views
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1Broad Institute,Massachusetts Institute of Technology and Harvard Medical School, 2Dept. of Biological Engineering,Massachusetts Institute of Technology

Summary

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Dieses Protokoll zeigt einen einfache, robuste und hohen Durchsatz Einzelmolekül Fluss-stretching Assay für das Studium eindimensionale (1D) Diffusion von Molekülen entlang DNA.

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Cite this Article

Xiong, K., Blainey, P. C. A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA. J. Vis. Exp. (128), e55923, doi:10.3791/55923 (2017).

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