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Análise abrangente de metilação de DNA usando um método baseado em captação de domínio de ligação de metil-CpG em pacientes com leucemia linfocítica crônica
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JoVE Journal Genetics
Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Análise abrangente de metilação de DNA usando um método baseado em captação de domínio de ligação de metil-CpG em pacientes com leucemia linfocítica crônica

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9,697 Views

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13:21 min

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June 16, 2017

DOI:

10.3791/55773-v

DOI:
10.3791/55773-v

•

13:21 min
June 16, 2017

•

4 Views
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1Department of Medical Genetics, Institute of Biomedicine,Gothenburg University, 2Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine,Gothenburg University

Summary

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Este trabalho descreve um protocolo optimizado de sequenciação de domínio de ligação de metil-CpG (MBD) e uma tubagem computacional para identificar regiões ricas em CpG diferencialmente metiladas em pacientes com leucemia linfocítica crônica (CLL).

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Cite this Article

Subhash, S., Kanduri, M. Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients. J. Vis. Exp. (124), e55773, doi:10.3791/55773 (2017).

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