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慢性リンパ球性白血病患者におけるメチル-CpG結合ドメイン捕捉に基づく包括的DNAメチル化解析

JoVE Journal
Genetics

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JoVE Journal Genetics

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

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慢性リンパ球性白血病患者におけるメチル-CpG結合ドメイン捕捉に基づく包括的DNAメチル化解析

9,697 Views

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13:21 min

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June 16, 2017

DOI:

10.3791/55773-v

DOI:

10.3791/55773-v

•

13:21 min

June 16, 2017

•

4 Views

Santhilal Subhash¹, Meena Kanduri²

¹Department of Medical Genetics, Institute of Biomedicine,Gothenburg University, ²Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine,Gothenburg University

Summary

この研究では、最適化されたメチル-CpG結合ドメイン（MBD）配列決定プロトコルと、慢性リンパ球性白血病（CLL）患者において差別的にメチル化されたCpGリッチ領域を同定するための計算パイプラインについて記載する。

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