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Analisi completa della metilazione del DNA usando un metodo basato sulla cattura di dominio di metile-CpG in pazienti con leucemia linfocitica cronica
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Genetics
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JoVE Journal Genetics
Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
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Analisi completa della metilazione del DNA usando un metodo basato sulla cattura di dominio di metile-CpG in pazienti con leucemia linfocitica cronica

italiano

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9,697 Views

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13:21 min

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June 16, 2017

DOI:

10.3791/55773-v

DOI:
10.3791/55773-v

•

13:21 min
June 16, 2017

•

4 Views
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1Department of Medical Genetics, Institute of Biomedicine,Gothenburg University, 2Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine,Gothenburg University

Summary

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Questo lavoro descrive un protocollo di sequenziamento del dominio di binding di metil-CpG (MBD) ottimizzato e una pipeline computazionale per identificare le regioni ricche di CpG riccamente differenziate in pazienti con leucemia linfocitaria cronica (CLL).

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Cite this Article

Subhash, S., Kanduri, M. Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients. J. Vis. Exp. (124), e55773, doi:10.3791/55773 (2017).

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