Journal
/
Biochemistry
/
Biyotinile Hücre Yüzey Proteinlerinin Arındırılması<emRhipicephalus microplus</em> Epitelyal Gut Hücreleri
JoVE Journal
Biochemistry
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
Please note that all translations are automatically generated. Click here for the English version.

Biyotinile Hücre Yüzey Proteinlerinin Arındırılması<emRhipicephalus microplus</em> Epitelyal Gut Hücreleri

Türkçe

Automatically Generated

9,278 Views

•

12:03 min

•

July 23, 2017

DOI:

10.3791/55747-v

DOI:
10.3791/55747-v

•

12:03 min
July 23, 2017

•

3 Views
, ,
1Queensland Alliance for Agriculture & Food Innovation,The University of Queensland, 2Centre for Comparative Genomics,Murdoch University

Summary

Automatically generated

Rhipicephalus microplus bağırsak dokusundan epitel hücrelerini izole etmek için modifiye bir yoğunluk santrifüj gradyan tabanlı metodoloji kullanıldı. Yüzeye bağlı proteinler, biyotinlenmiş ve streptavidin manyetik boncuklarla arıtılmış uygulamalarda kullanılmak üzere saflaştırılmıştır.

Explore More Videos

Biotinylated Cell Surface ProteinsRhipicephalus MicroplusEpithelial Gut CellsPercoll GradientCell DisassociationCell PurificationStreptavidin Magnetic Beads

Read Article

Cite this Article

Karbanowicz, T. P., Lew-Tabor, A., Rodriguez Valle, M. Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells. J. Vis. Exp. (125), e55747, doi:10.3791/55747 (2017).

Download .ris file

Copy

Share Video

.

Copy