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用于估计电惊厥治疗大鼠模型中细胞数的光学分级技术
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
The Optical Fractionator Technique to Estimate Cell Numbers in a Rat Model of Electroconvulsive Therapy
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用于估计电惊厥治疗大鼠模型中细胞数的光学分级技术

English

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11,259 Views

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07:55 min

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July 09, 2017

DOI:

10.3791/55737-v

DOI:
10.3791/55737-v

•

07:55 min
July 09, 2017

•

5 Views
, ,
1Research Laboratory for Stereology and Neuroscience,Bispebjerg-Frederiksberg Hospital, 2Institute of Clinical Medicine, Department of Health and Medical Sciences,University of Copenhagen

Summary

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在这里，我们提出了一种立体学方法，光学分级器，用于量化新的神经元的形成，以及它们的生存，在大脑海马中的电惊厥刺激。当正确实施时，立体感方法的灵敏度和效率确保了具有固定和预定精度的精确估计。

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Cite this Article

Olesen, M. V., Needham, E. K., Pakkenberg, B. The Optical Fractionator Technique to Estimate Cell Numbers in a Rat Model of Electroconvulsive Therapy. J. Vis. Exp. (125), e55737, doi:10.3791/55737 (2017).

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