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脳膜分画：<em> Ex Vivo</em> Subsynaptic Protein Localizationを評価するアプローチ
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Biochemistry
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JoVE Journal Biochemistry
Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization
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脳膜分画：<em> Ex Vivo</em> Subsynaptic Protein Localizationを評価するアプローチ

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10,222 Views

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09:49 min

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May 12, 2017

DOI:

10.3791/55661-v

DOI:
10.3791/55661-v

•

09:49 min
May 12, 2017

•

1 Views
, , , ,
1Unitat de Farmacologia, Departament Patologia i Terapèutica Experimental, Facultat de Medicina, IDIBELL,Universitat de Barcelona, L’Hospitalet de Llobregat, 2Institut de Neurociències,Universitat de Barcelona, 3Center for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine,University of Coimbra

Summary

Automatically generated

ここでは、異なるシナプスコンパートメントに属するタンパク質を単離するための堅牢な手順を表す脳膜分画プロトコルを提示します。

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Cite this Article

Morató, X., López-Cano, M., Canas, P. M., Cunha, R. A., Ciruela, F. Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization. J. Vis. Exp. (123), e55661, doi:10.3791/55661 (2017).

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