Journal
/
Developmental Biology
/
In Ovo Electroporation in de Kip Auditory Brainstem
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
In Ovo Electroporation in the Chicken Auditory Brainstem
Please note that all translations are automatically generated. Click here for the English version.

In Ovo Electroporation in de Kip Auditory Brainstem

Nederlands

Automatically Generated

8,291 Views

•

10:14 min

•

June 09, 2017

DOI:

10.3791/55628-v

DOI:
10.3791/55628-v

•

10:14 min
June 09, 2017

•

1 Views
, ,
1Roxelyn and Richard Pepper Department of Communication Sciences and Disorders,Northwestern University, 2Knowles Hearing Research Center,Northwestern University, 3Department of Neurobiology,Northwestern University

Summary

Automatically generated

Auditieve hersenstam neuronen van avians en zoogdieren zijn gespecialiseerd in snelle neurale codering, een fundamenteel proces voor normale gehoorfuncties. Deze neuronen ontstaan ​​uit duidelijke precursoren van embryonale hindbrain. We presenteren technieken die elektroporatie gebruiken om genen uit te drukken in het achterhoofd van kippenembryo's om de genfunctie tijdens de auditieve ontwikkeling te bestuderen.

Explore More Videos

In Ovo ElectroporationChicken Auditory BrainstemGene ExpressionAuditory Neuron DevelopmentPlasmid SolutionMicropipette PullerPicospritzerHamburger-Hamilton StageEgg IncubationEggshell Membrane RemovalAlbumin Withdrawal

Read Article

Cite this Article

Lu, T., Cohen, A. L., Sanchez, J. T. In Ovo Electroporation in the Chicken Auditory Brainstem. J. Vis. Exp. (124), e55628, doi:10.3791/55628 (2017).

Download .ris file

Copy

Share Video

.

Copy