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Analyzing Dendritic Morphology in Columns and Layers
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Neuroscience
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JoVE Journal Neuroscience
Analyzing Dendritic Morphology in Columns and Layers

Analyzing Dendritic Morphology in Columns and Layers

DOI:
10.3791/55410-v

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08:41 min

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March 23, 2017

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, , , , ,
1Section on Neuronal Connectivity, Eunice Kennedy Shriver National Institute of Child Health and Human Development,National Institutes of Health (NIH), 2Mathematical and Statistical Computing Laboratory, Center for Information Technology,National Institutes of Health (NIH), 3Biomedical Imaging Research Services Section, Center for Information Technology,National Institutes of Health (NIH)

Chapters

  • 00:05Title
  • 00:44Dual-image Acquisition
  • 03:11Neurite Tracing and Reference Point Assignment
  • 06:49Results: Comparison of Tm20 and Tm2 Neuron Morphology
  • 08:04Conclusion

Summary

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Here, we show how to analyze dendritic routing of Drosophila medulla neurons in columns and layers. The workflow includes a dual-view imaging technique to improve the image quality and computational tools for tracing, registering dendritic arbors to the reference column array and for analyzing the dendritic structures in 3D space.

Tags

Dendritic MorphologyDrosophila Medulla NeuronsColumnsLayersNeuroscienceDendritic WiringImagingConfocal MicroscopyGFP LabelingBrain ClearingSample Preparation

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