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רקומביננטי α-β - ו γ-Synucleins להמריץ את פעילות החלבון פוספטאז 2A קטליטי יחידת משנה של מבחני חינם תא
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Neuroscience
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JoVE Journal Neuroscience
Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays
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רקומביננטי α-β - ו γ-Synucleins להמריץ את פעילות החלבון פוספטאז 2A קטליטי יחידת משנה של מבחני חינם תא

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09:36 min

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August 13, 2017

DOI:

10.3791/55361-v

DOI:
10.3791/55361-v

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09:36 min
August 13, 2017

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1Department of Biomedical Sciences, Center of Emphasis in Neurosciences, Graduate School of Biomedical Sciences, Paul L. Foster School of Medicine,Texas Tech University Health Sciences Center El Paso

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Recombinant SynucleinProtein Phosphatase 2ACell-free AssayP-Nitrophenol Phosphate (pNPP) BufferMalachite Green SolutionTween 20 ActivatorThreonine PhosphopeptidePhosphate Standards

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Lek, S., Vargas-Medrano, J., Villanueva, E., Marcus, B. S., Godfrey, W., Perez, R. G. Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays. J. Vis. Exp. (126), e55361, doi:10.3791/55361 (2017).

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