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셀 마이그레이션 연구에서 수직 화학 산소 그라디언트에 대한 폴리 디메틸 실록산 - 폴리 카보네이트 미세 유체 장치
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients
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셀 마이그레이션 연구에서 수직 화학 산소 그라디언트에 대한 폴리 디메틸 실록산 - 폴리 카보네이트 미세 유체 장치

English

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13,802 Views

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11:23 min

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February 23, 2017

DOI:

10.3791/55292-v

DOI:
10.3791/55292-v

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11:23 min
February 23, 2017

•

4 Views
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1Research Center for Applied Sciences,Academia Sinica

Summary

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The control of chemical and oxygen gradients is essential for cell cultures. This paper reports a polydimethylsiloxane-polycarbonate (PDMS-PC) microfluidic device capable of reliably generating combinations of chemical and oxygen gradients for cell migration studies, which can be practically utilized in biological labs without sophisticated instrumentation.

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Cite this Article

Chiang, H., Yeh, S., Peng, C., Liao, W., Tung, Y. Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients. J. Vis. Exp. (120), e55292, doi:10.3791/55292 (2017).

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