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Visualisatie van eiwit-eiwit interacties in nucleaire en cytoplasmatische fracties door Co-immunoprecipitatie en<em> In Situ</em> Proximity Ligatie Assay
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Biochemistry
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JoVE Journal Biochemistry
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
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Visualisatie van eiwit-eiwit interacties in nucleaire en cytoplasmatische fracties door Co-immunoprecipitatie en<em> In Situ</em> Proximity Ligatie Assay

12,467 Views

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10:05 min

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January 16, 2017

DOI:

10.3791/55218-v

DOI:
10.3791/55218-v

•

10:05 min
January 16, 2017

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4 Views
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1University Children’s Hospital Basel (UKBB),University of Basel, 2Department of Clinical Research,University of Basel

Tags

Protein-protein InteractionCo-immunoprecipitationIn Situ Proximity Ligation AssayNuclear FractionCytoplasmic FractionCellular CompartmentsHEK293 CellsCytoplasmic ExtractionNuclear ExtractionProtein G Magnetic BeadsInput Samples

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Zhu, X., Zelmer, A., Wellmann, S. Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay. J. Vis. Exp. (119), e55218, doi:10.3791/55218 (2017).

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