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QTLマッピングとCRISPR / Cas9編集による薬剤耐性遺伝子同定<em> Toxoplasma gondii</em
JoVE Journal
Genetics
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JoVE Journal Genetics
QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
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QTLマッピングとCRISPR / Cas9編集による薬剤耐性遺伝子同定<em> Toxoplasma gondii</em

日本語

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15,918 Views

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11:37 min

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June 22, 2017

DOI:

10.3791/55185-v

DOI:
10.3791/55185-v

•

11:37 min
June 22, 2017

•

9 Views
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1State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine,Huazhong Agricultural University, 2Pathobiological Sciences, School of Veterinary Medicine,Louisiana State University

Summary

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Toxoplasma gondiiの薬剤耐性遺伝子を同定するために全ゲノム配列ベースの遺伝地図を用いたQTLマッピングをどのように使用することができるか、そしてこれがどのようにしてゲノム標的を効率的に編集するCRISPR / Cas9システム薬剤耐性遺伝子。

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Shen, B., Powell, R. H., Behnke, M. S. QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii. J. Vis. Exp. (124), e55185, doi:10.3791/55185 (2017).

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