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在从幼稚的CD4 + T细胞的人CD4 + FOXP3 +诱导调节性T细胞（iTregs的）使用含有TGF-β-协议的体外分化
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
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在从幼稚的CD4 + T细胞的人CD4 + FOXP3 +诱导调节性T细胞（iTregs的）使用含有TGF-β-协议的体外分化

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08:20 min

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December 30, 2016

DOI:

10.3791/55015-v

DOI:
10.3791/55015-v

•

08:20 min
December 30, 2016

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1Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna,Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory

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In VitroDifferentiationHumanCD4+FOXP3+Induced Regulatory T CellsITregsNaive CD4+ T CellsTGF-βPhenotypeT Cell ImmunologyPBMCMagnetic Bead IsolationBiotin AntibodyAnti-biotin MicrobeadsLS Column

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Schmidt, A., Éliás, S., Joshi, R. N., Tegnér, J. In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol. J. Vis. Exp. (118), e55015, doi:10.3791/55015 (2016).

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