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对于神经化学标记用biocytin填充和加工部分的免疫染色
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Neuroscience
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JoVE Journal Neuroscience
Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers
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对于神经化学标记用biocytin填充和加工部分的免疫染色

English

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22,468 Views

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08:54 min

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December 31, 2016

DOI:

10.3791/54880-v

DOI:
10.3791/54880-v

•

08:54 min
December 31, 2016

•

29 Views
, , ,
1Graduate School of Biomedical Sciences,Rutgers New Jersey Medical School, 2Department of Pharmacology, Physiology and Neuroscience,Rutgers New Jersey Medical School

Summary

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该协议提出了在使用生物胞素填充和随后的免疫组织化学后处理电生理记录修补神经元的形态回收的方法。我们表明，该染色和盖片厚生物胞素填充部分可以与第二初级抗体天或数月后进行复染。

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Cite this Article

Swietek, B., Gupta, A., Proddutur, A., Santhakumar, V. Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers. J. Vis. Exp. (118), e54880, doi:10.3791/54880 (2016).

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